Nucleotide sequence of the intergenic spacer and the 18S ribosomal RNA gene from mustard (Sinapis alba).

From a rRNA repeating unit (9.1 kbp) of mustard we have The DNA was sequenced according to the method of Maxam sequenced the first internal transcribed spacer, the 5.8S rRNA and Gilbert gene, and the second internal transcribed spacer [1]. Here, we present the nucleotide sequence of a 5500 bp fragment containing the 3 ' end terminus of the 25S gene (486 bp), the intergenic spacer REFERENCES (3204 bp), the 18S gene (1804 bp), and the first 6 nucleotides , R ^ ^ ^ c ^ ^ ( 1 9 8 9 ) Nucl Acids ^ 17, 7 5 2 2 of the first internal transcribed spacer. The coding regions have 2 Takmwa.F , Oono.K and Sugmra.M (1985) Plant Mol Biol 4,335-364 been determined in homology to the equivalent genes of rice [2]. 3 Maxam.A M. and Gilbert.w (1980) Methods m Enzymology 65,499-560